Methods included
– Sequencing by peptide mapping with LC-ESI-MS/MS
– Oxidation by peptide mapping with LC-ESI-MS/MS
– Deamination by peptide mapping with LC-ESI-MS/MS
– Isomerized aspartate by peptide mapping with LC-ESI-MS/MS
– N-glycan profiling with HILIC-FLD-MS of released and fluorescence labelled N-glycans
– Charge pattern by capillary isoelectric focusing (cIEF)
– Purity determined by SDS-capillary gel electrophoresis (CGE)
– Fragments and aggregates performed by size-exclusion chromatography (SEC)
