The originator molecule is analysed by peptide mapping with LC-ESI-MS/MS after proteolytic digestion with different proteases. The mass spectrometric data is matched to a patent database to verify the full sequence of the molecule with the Mascot search engine.
The originator molecule is analysed by peptide mapping with LC-ESI-MS/MS after proteolytic digestion with different proteases. Peptides containing oxidized methionine are identified and quantified against the respective non-modified peptide. The relative degree of oxidation for each modified methionine is summarized.
The originator molecule is analysed by peptide mapping with LC-ESI-MS/MS after proteolytic digestion with different proteases. Peptides containing deamidated asparagine or glutamine are identified and quantified against the respective non-modified peptide. The relative degree of deamidation for each modified asparagine or glutamine is summarized. Exact identification of modification site for neighbouring amino acids in some cases not possible.
The originator molecule is analysed by peptide mapping with LC-ESI-MS/MS after proteolytic digestion with different proteases. Peptides containing isomerized aspartate are identified and quantified against the respective non-modified peptide. The relative degree of isomerization for each modified aspartate is summarized. Exact identification of modification site for neighbouring amino acids in some cases not possible.
N-glycan profiling with HILIC-FLD-MS of released and fluorescence labelled N-glycans. The release is performed with Glycosidase F. The result contains the list of identified N-glycans and the relative distribution based on fluorescence or mass spectrometric signal).