Comprehensive Analytical Data Services for Biologic Characterization
Access precise, reliable molecular insights to accelerate your drug development programs.
About our Analytical Data Services
We provide a full suite of advanced analytical data to characterize biologic molecules with high accuracy. Partnering with a leading (bio)analytical CRO, we offer cutting-edge techniques such as N-glycosylation profiling and peptide sequencing.
Our Standard Analytical Data Services
Sequencing by Peptide Mapping (LC-ESI-MS/MS)
Peptide mapping after proteolytic digestion with multiple proteases is performed on the originator molecule. Mass spectrometry data is matched against patent databases using the Mascot search engine to verify the full protein sequence.
Oxidation Analysis by Peptide Mapping (LC-ESI-MS/MS)
Oxidized methionine-containing peptides are identified and quantified relative to their unmodified counterparts to determine the oxidation level for each site.
Deamidation Analysis by Peptide Mapping (LC-ESI-MS/MS)
Peptides with deamidated asparagine or glutamine residues are identified and quantified. The degree of deamidation is summarized for each site, though precise identification of neighboring amino acid modifications may sometimes be limited.
Isomerized Aspartate Analysis by Peptide Mapping (LC-ESI- MS/MS)
Peptides containing isomerized aspartate are identified and
quantified, with a summary of isomerization levels per site. Precise localization near neighboring amino acids may occasionally be limited.
N-Glycan Profiling (HILIC-FLD-MS)
Released and fluorescence-labeled N-glycans are profiled using hydrophilic interaction liquid chromatography with fluorescence and mass spectrometry detection. Glycosidase F is used for glycan release. Results include identified N-glycans and their relative abundance.
Charge Heterogeneity by Capillary Isoelectric Focusing (cIEF)
Charge variants of the molecule are separated by cIEF, revealing the charge pattern and isoelectric point (pI), which may reflect post-translational modifications.
Purity by SDS-Capillary Gel Electrophoresis (CGE)
CGE determines molecular purity before and after reduction (for dimeric proteins like antibodies) and detects protein fragments and impurities.
Fragment and Aggregate Analysis by Size-Exclusion Chromatography (SEC)
SEC separates protein isoforms by molecular weight, allowing
quantification of monomers, fragments, and aggregates.
Our Packages
Comprehensive Package
Includes all 8 standard analytical methods for a complete molecular profile.
Individual Package
Select only the analytical methods relevant to your project needs.
Tailored Package
Customize your analysis with methods beyond our standard offerings—let us know your specific requirements.
