analytical-services

Analytical data service

Now, you can access a comprehensive suite of analytical methods to analyze biologic molecules. For a variety of methods including N-glicolisation and sequencing, we have teamed up with a leading (bio)analytical CRO. By providing you with relevant and precise information about the molecules you are working with, our technology can support your drug development programs.

Explore the 3 different analytical solutions

Edit

Comprehensive package​

This option includes our 8 standard analytical methods

Methods included​

– Sequencing by peptide mapping with LC-ESI-MS/MS
– Oxidation by peptide mapping with LC-ESI-MS/MS
– Deamination by peptide mapping with LC-ESI-MS/MS
– Isomerized aspartate by peptide mapping with LC-ESI-MS/MS
– N-glycan profiling with HILIC-FLD-MS of released and fluorescence labelled N-glycans
– Charge pattern by capillary isoelectric focusing (cIEF)
– Purity determined by SDS-capillary gel electrophoresis (CGE)
– Fragments and aggregates performed by size-exclusion chromatography (SEC)

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Edit

Individual analysis package

You can access each of the methods individually

request a quote + download a free sample​

1. Select a molecule and choose your method(s) of interest
2. Provide your contact details
3. Send us your request and download a free sample report


Please provide your contact information


send us your inquiry and download a free sample file


Edit

Taylored package

This will give you the chance to choose methods other then the standard ones already mentioned.

request a quote + download a free sample​

1. Select a molecule
2. Provide your contact details
3. Send us your request and download a free sample report


Please provide your contact information


send us your inquiry and download a free sample file


The characterizing process of a molecule is useful in a number of stages of its development, including:

About our standard analytical services

Sequencing by peptide mapping with LC-ESI-MS/MS

The originator molecule is analysed by peptide mapping with LC-ESI-MS/MS after proteolytic digestion with different proteases. The mass spectrometric data is matched to a patent database to verify the full sequence of the molecule with the Mascot search engine.

Oxidation by peptide mapping with LC-ESI-MS/MS

The originator molecule is analysed by peptide mapping with LC-ESI-MS/MS after proteolytic digestion with different proteases. Peptides containing oxidized methionine are identified and quantified against the respective non-modified peptide. The relative degree of oxidation for each modified methionine is summarized.

Deamination by peptide mapping with LC-ESI-MS/MS

The originator molecule is analysed by peptide mapping with LC-ESI-MS/MS after proteolytic digestion with different proteases. Peptides containing deamidated asparagine or glutamine are identified and quantified against the respective non-modified peptide. The relative degree of deamidation for each modified asparagine or glutamine is summarized. Exact identification of modification site for neighbouring amino acids in some cases not possible.

Isomerized aspartate by peptide mapping with LC-ESI-MS/MS

The originator molecule is analysed by peptide mapping with LC-ESI-MS/MS after proteolytic digestion with different proteases. Peptides containing isomerized aspartate are identified and quantified against the respective non-modified peptide. The relative degree of isomerization for each modified aspartate is summarized. Exact identification of modification site for neighbouring amino acids in some cases not possible.

N-glycan profiling with HILIC-FLD-MS of released and fluorescence labelled N-glycans

N-glycan profiling with HILIC-FLD-MS of released and fluorescence labelled N-glycans. The release is performed with Glycosidase F. The result contains the list of identified N-glycans and the relative distribution based on fluorescence or mass spectrometric signal).

Charge pattern by capillary isoelectric focusing (cIEF)

The charge heterogeneity profile of an originator molecule is analysed by capillary isoelectric focusing (cIEF). Based on the separation in a capillary gel electrophoresis system the charge pattern (due to for example post-translational modifications), as well as the isoelectric point (pI) of the protein,pI is determined.

Purity determined by SDS-capillary gel electrophoresis (CGE)

The purity of an originator molecule can be determined by SDS-capillary gel electrophoresis (CGE). CGE analysis can be performed before and after reduction of dimeric proteins such as antibodies and generates data of protein fragments and impurities.

Fragments and aggregates performed by size-exclusion chromatography (SEC)

Aggregate analysis of an originator molecule is performed by size-exclusion chromatography (SEC). Based on the chromatographic method the protein isoforms are separated by their molecular weight and thereby allowing the determination and quantification of monomer, fragments and aggregates.
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